antibodies rabbit anti stmn2 scg10 novus biologicals  (Novus Biologicals)

Journal: eLife

Article Title: Endothelin B receptor inhibition rescues aging-dependent neuronal regenerative decline

doi: 10.7554/eLife.100217

Figure Lengend Snippet: ( A ) Scheme of drug treatment and peripheral nerve injury model. ( B ) Quantification of the length of the 10 longest axons in indicated conditions. ( C ) Quantification of the regeneration index, calculated as the distance along the nerve where the SGC10 intensity is 50% of the SCG10 intensity at crush site. ( D ) Representative longitudinal sections of sciatic nerves 24 h after SNC, immunostained for SCG10, from mice with the indicated treatment. Dotted line indicates the crush site, determined as the maximal SGC10 intensity (scale bars, 200 μm). ( E ) Quantification of SCG10 intensity at the indicated distance normalized to the intensity at the crush site for each condition. N=5 mice/condition. ( F ) Scheme of long-term Bosentan treatment. ( G ) Representative images of hindpaw skin after long-term Bosentan treatment immunostained for PGP9.5 (white) and DAPI (blue) (scale bars, 50 μm). ( H ) Quantification of intraepidermal nerve fiber density (IENFD) 24 days after sciatic nerve crush from the indicated groups. N=5 mice/condition. ( I ) Scheme of adult DRG neuronal culture and treatments. ( J, L ) Representative images showing TUJ1 (black) immunostaining of neurons in DRG cultures (scale bars, 100 μm). ( K, M ) Quantification of axonal radial length ( K ) and total TUJ1 + area ( L ). Different colors represent different biological replicates. N (neuron number)=177 (vehicle; three biological replicates, naïve mice), 168 (Ambrisentan, three biological replicates, naïve mice), 183 (Bosentan; three biological replicates, naïve mice), 186 (vehicle; three biological replicates, injured mice), 204 (Ambrisentan, three biological replicates, injured mice), and 210 (Bosentan; three biological replicates, injured mice), respectively. The data are presented as mean ± SD.

Article Snippet: Antibody , Rabbit anti-STMN2 (SCG10) (polyclonal) , Novus/Techne , NBP1-49461 RRID: AB_10011569 , IHC (1:1000).

Techniques: Immunostaining

Journal: eLife

Article Title: Endothelin B receptor inhibition rescues aging-dependent neuronal regenerative decline

doi: 10.7554/eLife.100217

Figure Lengend Snippet: ( A ) Scheme of drug treatment and peripheral nerve injury model. ( B ) Quantification of the 10 longest axons in indicated groups. ( C ) Quantification of 50% regenerative index, calculated as the distance along the nerve where the SCG10 intensity is 50% of the SCG10 intensity at crush site. ( D ) Representative longitudinal sections of sciatic nerves 3 d after SNC immunostained for SCG10 from mice with the indicated treatment. Dotted line indicates the crush site, determined as the maximal SCG10 intensity (scale bars, 200 μm). ( E ) Quantification of SCG10 intensity at the indicated distance normalized to the intensity at the crush site for each condition. N=5 mice/condition. The data are presented as mean ± SD. ( F ) RT-qPCR of Atf3, Aif1, Fabp7, Edn1, Ednra, and Ednrb gene in contralateral (CON) and ipsilateral DRGs at 3 days post injury (SNC). N (mouse number)=4/each group. ( G ) Western blot analysis and quantification of ETBR protein expression in DRGs from the mice with/without injury. ( H ) Quantification of ETBR expression normalized to GAPDH expression (folder of control mice). N (mouse number)=8/group. The data are presented as mean ± SD. Figure 3—figure supplement 1—source data 1. Original files for western blot analysis. Figure 3—figure supplement 1—source data 2. PDF file containing original western blots, indicating the relevant bands and treatments.

Article Snippet: Antibody , Rabbit anti-STMN2 (SCG10) (polyclonal) , Novus/Techne , NBP1-49461 RRID: AB_10011569 , IHC (1:1000).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Control

Journal: eLife

Article Title: Endothelin B receptor inhibition rescues aging-dependent neuronal regenerative decline

doi: 10.7554/eLife.100217

Figure Lengend Snippet: ( A ) Scheme of drug treatment and dorsal root crush injury model. ( B ) Representative longitudinal sections of sciatic nerves 3 d after DRC immunostained for SCG10 from mice with the indicated treatment. Dotted line indicates the crush site, determined as the maximal SCG10 intensity (scale bars, 200 μm). ( C ) Quantification of the 10 longest axons in indicated groups. ( D ) Quantification of 50% regenerative index, calculated as the distance along the nerve where the SCG10 intensity is 50% of the SCG10 intensity at crush site. ( E ) Quantification of SCG10 intensity at the indicated distance normalized to the intensity at the crush site for each condition. N=5 mice/condition. The data are presented as mean ± SD.

Article Snippet: Antibody , Rabbit anti-STMN2 (SCG10) (polyclonal) , Novus/Techne , NBP1-49461 RRID: AB_10011569 , IHC (1:1000).

Techniques:

Journal: eLife

Article Title: Endothelin B receptor inhibition rescues aging-dependent neuronal regenerative decline

doi: 10.7554/eLife.100217

Figure Lengend Snippet: ( A ) Scheme of drug treatment and DRG explant model. ( B ) Representative images of DRG explants 7 days after drug treatment (scale bars, 1000 μm). ( C ) Quantification of radial length of the 50 longest axons from DRG explants. N=36 explants from 6 individual mice (BQ788; 18 replicates, Veh; 18 replicates). ( D ) Representative longitudinal sections of sciatic nerves 3 d after SNC immunostained for SCG10 from mice with the indicated treatment. Dotted line indicates the crush site, determined as the maximal SCG10 intensity (scale bars, 200 μm). ( E, F ) Quantification of the 10 longest axons in indicated groups ( E ). Quantification of 50% regenerative index, calculated as the distance along the nerve where the SCG10 intensity is 50% of the SCG10 intensity at crush site ( F ). ( G ) Quantification of the SCG10 intensity at the indicated distance normalized to the intensity at the crush site for each condition. N (mouse number) = 4(adult, vehicle), 3 (aged, vehicle), and 3 (Bosentan +Age), respectively. The data are presented as mean ± SD.

Article Snippet: Antibody , Rabbit anti-STMN2 (SCG10) (polyclonal) , Novus/Techne , NBP1-49461 RRID: AB_10011569 , IHC (1:1000).

Techniques: